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Molecular basis for the enzymatic inactivity of class III glutaredoxin ROXY9 on normal glutathionylated substrates

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, Virtually no information and facts is readily available for class III GRXs. This has long been as a result of encountered complications when purifying recombinant proteins expressed in E. coli30. Right here, we succeeded in acquiring milligram quantities of course III GRX ROXY9 from Arabidopsis thaliana by implementing the baculovirus expression system in insect cells.

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The colour code from the triangles corresponds on the colour code from the redox condition as based on mass spectrometry. Molecular masses of marker proteins (M) are indicated in kDa. (b, f) Relative depth proportions of peptides containing the Energetic web page Together with the indicated modifications. The results are from 3 or 4 replicates, with Each and every replicate symbolizing an independent treatment method. Resource info are delivered as being a Supply Details file.